Therefore, these findings suggest that the LentiCRISPRv2/dCas9‐TET1CD‐sgRNAs demethylation system enhances the inhibitory effects on the proliferation, migration and invasion of NPC cells by transcriptionally activating BRD7 expression through targeted demethylation of BRD7 promoter, thus exerting a dramatic tumour inhibitory effect in NPC in vitro. The gene discussed is BRD7; the disease is nasopharyngeal carcinoma.