In the current study, through integrating proteomic profiling, chromatin immunoprecipitation sequencing (ChIP-seq), and transcriptome-wide characterization, we discover that armadillo repeat containing 12 (ARMC12) serves as a co-factor of transcription factor MYC to drive the expression of nucleoporin-encoding genes (NUP62, NUP93, and NUP98), leading to elevation in NPC number and nuclear translocation of transcriptional regulators, such as AMRC12 15, MYC 16, cut like homeobox 1 (CUX1) 17, or E2F transcription factor 1 (E2F1) 18, and invasion or metastatic dissemination of NB cells. This evidence concerns the gene E2F1 and neuroblastoma.