In vivo, CAMK1D interference reduced Treg infiltration and elevated CD8+ T cell levels from 41.87% to 54.89%, accompanied by a substantial increase in IFN-γ+ CD8+ T cells from 28.78% to 59.45% in PCa tissues (Figures 6E-G, 6K and S9). This evidence concerns the gene CD8A and posterior cortical atrophy.