After two vaccinations, splenocytes from ICC@G-PL-vaccinated mice co-cultured with tumour cells produced more IFN-γ and TNF-α spots (ELISPOT) and secreted higher levels of these cytokines (ELISA), indicating tumour-specific T cell development (Figure 4J-M). The gene discussed is IFNG; the disease is intrahepatic cholangiocarcinoma.