Like in ecDNA+ human tumor cells, ectopic expression of cGASWT in CT1BA5-EC cells produced cGAMP, although to a level lower than the histone-binding-deficient mutant cGASR236E, but substantially higher than the cGAMP level in CT1BA5-HSR cells that express similar levels of WT cGAS (Figure 3C, p = 0.0025). This evidence concerns the gene CGAS and neoplasm.