cGAS was not detected in the colorectal adenocarcinoma COLO320 isogenic cell lines—DM (ecDNA+) and HSR (ecDNA−), the glioblastoma GBM39 isogenic cell lines—KT (ecDNA+) and HSR (ecDNA−), or neuroblastoma ecDNA+ cell lines CHP212 and Kelly. This evidence concerns the gene CGAS and colorectal adenocarcinoma.