Recognizing the critical role of NOTCH in cell-cell interactions, we explored NOTCH signaling in our organoid model through gamma secretase inhibition and found remarkable cooperativity of NOTCH and YAP/TEAD in maintenance of the PRAD state, consistent with evidence that NOTCH can suppress NE lineage transitions in prostate and lung cancer models (34, 35). The gene discussed is YAP1; the disease is lung cancer.