Notably, we found a positive correlation between the infiltration level of CD20<sup>+</sup>FCRL4<sup>+</sup> B cells and the expression of PD-1, as well as the response to anti-PD-1 therapy or gemcitabine plus cisplatin combined with anti-PD-1 therapy in NPC.Overall, our study elucidates the potential anti-tumor mechanisms of CD20<sup>+</sup>FCRL4<sup>+</sup> B cells and provides insights into their role in immunotherapy for NPC. The gene discussed is FCRL4; the disease is neoplasm.