Western blot results revealed increased expression of key glycolytic enzymes (HK1 and PKM) and pyroptosis-related proteins (NLRP3, GSDMD-N, and Cleaved-caspase-1) in the calcified aortas of the mice in the CKD group compared with those in the aortas of the mice in the control group. This evidence concerns the gene NLRP3 and chronic kidney disease.