Western blot results revealed increased expression of key glycolytic enzymes (HK1 and PKM) and pyroptosis-related proteins (NLRP3, GSDMD-N, and Cleaved-caspase-1) in the calcified aortas of the mice in the CKD group compared with those in the aortas of the mice in the control group. The gene discussed is PKM; the disease is chronic kidney disease.