COL2A1 and cancer: Volcano plots showed significant up-regulation of cancer-associated genes, including extracellular matrix (ECM) components (Col6a1, Col2a1, Bgn, Acan, Cspg4, Vcan, Mgp, Mmp2), cell-cycle regulators (Ccnd3, Cdk4, Ccnd1), immune-related genes (Ifitm2, Ifitm3, B2m, Lgals1, H2-D1, Tnfrsf1a, Cd9, Gpnmb), and the complement component C4b in tumoral spots (Figures 1G, 1H, and S3E–S3H).