To evaluate the effect of CSRNP1 knockdown on apoptosis in HCC cells, we found that CSRNP1 knockdown significantly inhibited apoptosis in Hep3B and Huh7 cells, as determined by flow cytometry analysis, with apoptosis rates decreasing by approximately 55% and 60%, respectively (Fig. 6A,B). This evidence concerns the gene CSRNP1 and hepatocellular carcinoma.