Anti-tumor efficacy was further evaluated in LNP-circRNA<sup>E7E6</sup> vaccine combined with anti-PD-L1 antibody treatment.<h4>Results</h4>Prime-boost vaccination with LNP-circRNA<sup>E7E6</sup> induced a large pool of functional antigen-specific cytotoxic T cells in both the peripheral blood and spleen. Here, CD274 is linked to neoplasm.