Mechanistic studies, including RNA immunoprecipitation (RIP), ac4C RNA immunoprecipitation (acRIP), and co-immunoprecipitation (Co-IP), were conducted to elucidate the interaction between NAT10 and histone deacetylase 4 (HDAC4) and their roles in regulating NF-κB signaling and programmed death-ligand 1 (PD-L1) expression.<h4>Results</h4>NAT10 expression was significantly upregulated in breast cancer and correlated with poor patient prognosis. Here, HDAC4 is linked to breast cancer.