Consistent with prior reports we observed that bone marrow-derived macrophages (BMDMs) infected with L. interrogans serovar Copenhageni strain Fiocruz L1-130 at a multiplicity of infection (MOI) of 100 elicited the secretion of TNF, IL-10, and IL-6 (Fig 1A-1C) [30,31]. This evidence concerns the gene IL10 and infection.