Immunohistochemistry (IHC), Western blot, and quantitative real‐time polymerase chain reaction (qRT‐PCR) were used to detect the effects of NKILA on the Warburg effect, autophagy, programed cell death 1 ligand 1 (PD‐L1) expression, and CD8+ T cytotoxicity in ICC cells. This evidence concerns the gene CD274 and intrahepatic cholangiocarcinoma.