This study demonstrated that MTX1 overexpression activated the mTOR pathway to inhibit the autophagy proteins LC3II/LC3I and Beclin1, stimulate the Warburg effect, and upregulate glycolytic enzymes HK2, PKM2, and LDH, thus initiating the immune escape mechanism in ICC. The gene discussed is HK2; the disease is intrahepatic cholangiocarcinoma.