ENCORI analysis identified four NKILA‐binding RNAs, alpha‐1,3‐glucosyltransferase (ALG6), sushi domain containing 6 (SUSD6), actin filament associated protein 1 (AFAP1), and metaxin 1 (MTX1), which were differentially expressed in cholangiocarcinoma tissues compared to adjacent tissues in GEPIA analysis. The gene discussed is AFAP1; the disease is cholangiocarcinoma.