Brains from MA10-infected and control mice were immunofluorescently labeled for microglia (IBA1) and astrocytes (GFAP) to determine if there was an increase in glial activation in response to MA10 infection at day 21 p.i. We elected to examine the cortex (CRTX), dentate gyrus (DG), and subiculum (SUB) as previous studies of 5xFAD mice indicate changes in AD associated pathology in these regions 73. This evidence concerns the gene GFAP and infection.