We performed RNAseq analysis on A549 lung cancer cells and 122L and 153L (HMEC cultures derived from post-menopausal women), and 240L (HMEC cells derived from an 18-year-old woman) that had been treated with EGF (A549 only), TGF-β1 (HMEC only), or IL-6 (both A549 and HMEC) for six hours. This evidence concerns the gene TGFB1 and lung cancer.