In this study, it was showed that PRSS22 reduced the ANXA1 expression in the GC tumor model and the in vitro coculture model, and the inhibition of ANXA1 recovered the effects of si-PRSS22 on cell proliferation, migration, Edu positivity, LDH activity, and PI levels of GC and the immune evasion of T cells in the coculture model with GC cell and T cell. Here, PRSS22 is linked to gastric cancer.