ELISA results showed that serum GSH, SOD, GPX4, and SLC3A2 were decreased, and MDA, and ACSL4 were increased in CHF rats, and that Shenfu injection upregulated serum GSH, SOD, GPX4, and SLC3A2 levels and decreased MDA and ACSL4 levels in a dose-dependent manner (Figures 2B–2G). Here, GPX4 is linked to congestive heart failure.