In the present study, Shenfu injection removed excess ROS and Fe2+ from the cardiac tissues of yang-deficient CHF rats, decreased MDA and ACSL4, and increased SOD, GSH, GPX4, SLC3A2, and SLC7A11, while also increasing Nrf2 and decreasing Ptgs2 expression. This evidence concerns the gene ACSL4 and congestive heart failure.