AKT1 and congestive heart failure: Our results show that Shenfu injection induced Akt and GSK-3β phosphorylation and Nrf2 expression in a dose-dependent manner, and that the effect of Shenfu injection on the phosphorylation of GSK-3β and Nrf2 expression was inhibited by LY294002—weakening the therapeutic effect of Shenfu injection on cardiac tissue injury and cellular ferroptosis in yang-deficient CHF rats.