Greater S9.6 signal was seen in U251 and GBM6 cells when compared to fibroblasts and H1B.3 neural stem cells, respectively, supporting the notion that GBM may require THOC1-mediated deacetylation to avoid toxic R-loop accumulation (p<0.05) (Fig. 4O). The gene discussed is THOC1; the disease is glioblastoma.