A more comprehensive study used hiPSC from an AD patient (ε4 allele of APOE), and a healthy subject (ε3 allele of APOE) to differentiate iBMVECs, pericytes, astrocytes, microglia, and NPCs to produce multiple co-culture models of iBMVECs with individual cells using a transwell model. This evidence concerns the gene APOE and Alzheimer disease.