These findingswere confirmed in an independent cohort using qRT-PCR, which showedsignificantly higher expression of PGK1, ALDOA, and ENO2 in tumors compared to normalliver (Figure E).Immunohistochemistry data from the Human Protein Atlas further confirmedincreased protein levels of these enzymes in HCC samples (Figure F). This evidence concerns the gene PGK1 and hepatocellular carcinoma.