To dissect the role of GXYLT2 in GC cells, we stably knocked down its expression in the diffuse-subtype GC cell lines, including HGC-27, MKN1, and MKN45 cells, by two shRNAs targeting different regions of GXYLT2 mRNA (sh1 and sh2; Fig. 3A–C). The gene discussed is GXYLT2; the disease is gastric cancer.