RUNX1 depletion uniquely affected stemness programs, significantly downregulating haematopoietic stem cell signatures (NES range: −1.5 to −2.1, FDR < 0.05) while mildly enriching myeloid differentiation pathways (Figure 4E,F), consistent with its canonical role in sustaining immature AML cell states. This evidence concerns the gene RUNX1 and acute myeloid leukemia.