Notably, transcripts encoding the retromer proteins, VPS35 and VPS26B, which were not detected using conventional RNA-seq analyses of FTLD-TDP/ALS patient tissues (Fig 1A and 1B, first track) or TDP-43-depleted iNeurons (Fig 1A and 1B, second track), were readily identified when using the more sensitive 3′ end sequencing method (Fig 1A and 1B, third track). Here, VPS35 is linked to amyotrophic lateral sclerosis.