<h4>Background</h4>This study aimed to investigate the usefulness of time-dependent diffusion magnetic resonance imaging (MRI) parameters compared with the conventional apparent diffusion coefficient (ADC) in distinguishing tumor grade and isocitrate dehydrogenase (IDH) genotypes of adult-type diffuse gliomas.<h4>Methods</h4>This retrospective study included 102 patients with adult-type diffuse gliomas. This evidence concerns the gene IDH3A and neoplasm.