Likewise, in females with obesity (Figure 4L), vaccinated mice had significantly lower levels of eotaxin, GM-CSF, GRO-α, IL-1β, IL-2, IL-5, IL-6, IL-12p70, IL-17A, IL-18, IP-10, MCP-1, MCP-3, MIP-1α, MIP-1β, MIP-2α, and TNF-α compared to unvaccinated controls. This evidence concerns the gene IL1B and obesity due to melanocortin 4 receptor deficiency.