RNA-seq analysis of DU145 and C4–2 cells treated with palbociclib for 24 hours revealed significant activation of the cytosolic DNA-sensing pathway, cytokineingallyon receptor interactions, and Toll-like receptor signaling; in a murine RM-1 xenograft model, tumor volumes were significantly smaller following combination treatment with palbociclib and diABZI compared to monotherapies (n=5, p<0.001), with elevated infiltration of CD4+ and CD8+ T cells (n=3, p<0.001). This evidence concerns the gene CD8A and neoplasm.