Western blot analysis further confirmed that knockdown of LST1 led to a significant decrease in MHC-I protein expression in ACHN cells (Figures 6F, G, p<0.01), suggesting that LST1 may directly regulate the transcription or translation of MHC-I pathway-related genes, thereby reshaping intercellular communication patterns in T2DM and ccRCC. This evidence concerns the gene LST1 and nonpapillary renal cell carcinoma.