Through a mediation analysis, we found the effect of F11 on CES, SH3BGRL3 on AS and AIS, SPATA20 on SVS and SWAP70 on AIS were partially mediated by FXI, AF, T2D and SBP, respectively, demonstrating a critical role of these risk factors in the pathogenesis of stroke in consistence with previous epidemiological studies.30-34. Here, SPATA20 is linked to type 2 diabetes mellitus.