Although CRISPR-Cas9 knockout experiments confirmed the role of GNA15 in CRC cell proliferation, the current study lacks rescue experiments (e.g., restoring GNA15 expression in knockout cells to verify whether cell proliferation can be recovered) and pathway-specific inhibition experiments (e.g., inhibiting key molecules in the predicted pathways to observe changes in GNA15’s regulatory effects). The gene discussed is GNA15; the disease is colorectal carcinoma.