Our results suggest that, despite the activation of the TNF-α/NF-κB signaling pathway and high expression of IL-8 and IL-1β, the secretome of hAMSCs can promote apoptosis in Panc1 pancreatic cancer cells through the upregulation of p53, PUMA, and Caspase3. Here, IL1B is linked to familial pancreatic carcinoma.