MEST and neoplasm: ,38MiR-335 is located within an intron of MEST, a gene presenting hypermethylation of its promoter in HCC,39 which may explain the lower expression of miR-335-5p in tumor tissue compared to the surrounding nontumor tissue.37, 38, 39 Overexpression of miR-335-5p or application of miR-335-5p via exosomes decreased proliferation rates of hepatoma cells, both in vitro and in vivo.40