To explore whether betulinic acid (BA) influences FA pathway activity, three glioma cell lines with distinct genetic and pathological features were examined: U87MG (glioblastoma multiforme, PTEN mutant, wild‐type p53, poorly differentiated), LN229 (glioblastoma, wild‐type PTEN, p53 mutant, moderately differentiated), and U251 (glioblastoma, p53 mutant, highly proliferative). The gene discussed is TP53; the disease is glioma.