To explore whether betulinic acid (BA) influences FA pathway activity, three glioma cell lines with distinct genetic and pathological features were examined: U87MG (glioblastoma multiforme, PTEN mutant, wild‐type p53, poorly differentiated), LN229 (glioblastoma, wild‐type PTEN, p53 mutant, moderately differentiated), and U251 (glioblastoma, p53 mutant, highly proliferative). This evidence concerns the gene PTEN and central nervous system cancer.