To investigate the clinical significance of the DLEU1/ASCC2/ALKBH3/E2F1 signaling pathway in GC, we simultaneously examined the DLEU1 mRNA expression and the protein expression levels of ASCC2, ALKBH3 and E2F1 in tumor and normal gastric tissues using mIHC staining on tissue microarrays (TMA; N = 26, T = 104; Fig. 8A). This evidence concerns the gene E2F1 and gastric cancer.