While the magnitude of increase in IFNγ and CXCL10 in response to αCD3/αCD28 between cross-well and sequential treatment varied, the directionality of response was in high agreement with 5 of 6 tumor samples showing concordance for IFNγ and 6 of 6 for CXCL10 as measured using a > 1.5-fold change as an indicator of increased rate of cytokine production (Fig. 5C). Here, CXCL10 is linked to neoplasm.