To this end, the authors used melanoma B16-F10 cells with exquisite potential to colonize lymph nodes that were generated through serial in vivo selection by the Reticker-Flynn team, and found them to markedly upregulate FSP1 while exhibiting reduced protein levels of GPX4 and glutamate-cysteine ligase catalytic subunit (GCLC), which is required for the synthesis of glutathione, a key enzymatic co-factor for GPX4. This evidence concerns the gene GPX4 and melanoma.