At CKD progression equivalent to human CKD stages 4-5, we examined cardiac tissue for mitochondrial respiration, kidney histology for fibrosis, blood for BUN and CKD-MBD components, kidney tissue for klotho production, and aorta for VC.<h4>Results</h4>The HP diet produced hyperphosphatemia in the CKD animals compared with WT. Here, KL is linked to chronic kidney disease.