Next, we confirmed that macrophage-derived CXCL1/2 and CFB were upregulated in tumors, liver and lung tissues, as well as serum from mice orthotopically inoculated with 4T1 breast cancer cells after Dox treatment by immunofluorescence staining (Figure 3, F and G, and Supplemental Figure 6A) and ELISAs (Figure 3H). Here, CXCL1 is linked to breast cancer.