After chemotherapy application, we found 4T1 tumors with CTSC knockdown (shCtsc) grew much slower than those transfected with NC lentiviruses (shNC) (Figure 5E), and tumoral NET expression was inhibited in accordance (Figure 5F), suggesting CTSC in tumor debris worked as an important contributor to NETosis in postchemotherapy breast tumor. This evidence concerns the gene CTSC and breast neoplasm.