Immunohistochemical staining for the HCC marker, glypican 3 (GCP3) in livers harboring tumors, demonstrated positive staining in five of nine Casp2C320S and two of four Casp2−/− mice, while the two WT livers with tumor nodules were negative for GCP3, suggesting that these liver tumors were not HCC (Fig. 2G). This evidence concerns the gene GPC3 and hepatocellular carcinoma.