To test for a possible benefit of increasing O-GlcNAcylation on glial cell reactivity, we first used Western blot to determine if expression of GFAP, an astrocyte marker, and expression of Iba-1, a microglia marker, are increased in the DG of TgF344-AD rats, and if these levels are decreased by TMG treatment. Here, GFAP is linked to Alzheimer disease.