Selective depletion of RIG-I or TLR3 revealed RIG-I as the primary sensor mediating IFN-β induction upon ZIKV infection, consistent with studies conducted with various human cell types (Hamel et al., 2015; Singh et al., 2017; Hertzog et al., 2018; Schilling et al., 2020; Plociennikowska et al., 2021). Here, TLR3 is linked to Zika virus infectious disease.