Therefore, we speculate that the mechanism by which El1405 alleviates colitis may be by regulating the intestinal microbiota, significantly reducing the abundance of pathogens (especially E. faecalis and E. coli), reducing the damage to the intestinal barrier, thereby reducing the local and systemic levels of IL-17, IL-6, and TNF-α, and ultimately alleviating colitis. Here, TNF is linked to colitis.