CD86 and necrotizing enterocolitis: Immunofluorescence double‐labeling confirmed that F4/80+/CD86+ (Figure 4f,g) coexpression (type M1) was increased in the intestinal basal layer of the NEC group compared with that in the control group (p < 0.05), and the proportion of this colocalization continued to increase after the addition of succinate to the NEC group (p < 0.05), whereas although F4/80+/CD206+ (Figure 4h,i) coexpression (type M2) was elevated in the intestinal basal layer of the NEC group, its colocalization level could be decreased by the addition of succinate to the NEC group (p < 0.05).