To investigate whether succinate promotes the polarization of intestinal macrophages in NEC through the PI3K/AKT pathway, we evaluated the expression of macrophage markers and inflammatory factors using qRT‐PCR, western blot, immunofluorescence, and ELISA following the administration of the PI3K inhibitor LY294002 in vivo. This evidence concerns the gene AKT1 and necrotizing enterocolitis.