The heatmap showed that miRNA-4454 and miRNA-4516 were upregulated in glioma cells with SPHK2 knockdown, while miRNA-195-5p, miRNA-1273d, miRNA-3158-3p, miRNA-3689a-5p, miRNA-3689b-5p, miRNA-3689e, miRNA-548aa, miRNA-548t-3p, miRNA-548-5p, and miRNA-387e were downregulated in glioma cells with SPHK2 knockdown (Figure 2). This evidence concerns the gene SPHK2 and central nervous system cancer.