Colonization with DPM did not cause signs of enterocolitis (sleepiness, anorexia, fever, and diarrhea), histological changes in the intestine, the density of acid mucin‐producing cells, expression of villin, claudin‐1, occludin, TLR4, MD‐2, CD14, LBP, MyD88, and TRIF, induction of inflammatory cytokines IL‐1β, IL‐6, IL‐8, IL‐10, IL‐12/23p40, TNF‐α, IFN‐γ, and HMGB1. This evidence concerns the gene TNF and enterocolitis.