Regarding the integrated diagnosis based on WHO 2021 classification, glioblastoma, isocitrate dehydrogenase mutant astrocytoma grade 4, diffuse midline glioma H3-altered, and diffuse hemispheric glioma G34 mutant were present in 99 (85%), 10 (9%), 3 (3%), and 4 (3%) patients, respectively. The gene discussed is IDH3A; the disease is glioblastoma.