To interrogate the effect of Suv420h1 depletion on the TIME, flank parental and Suv420h1 KO MOC1 tumors were implanted, surgically resected (day 28 post-tumor implantation), digested into single cells and multicolor flow cytometry was conducted in the parental (NT, n=4–5) and Suv420h1 KO MOC1 tumors (KO-1, n=4; KO-2, n=3) (Fig. 4C, Supplementary Fig. 5). This evidence concerns the gene KMT5B and neoplasm.