CD8A and neoplasm: RNA velocity analysis on these data yielded a trajectory from naive CD8+ T cells (Cluster 4) to CXCL13+CD8+ T cells (Cluster 3) to cytotoxic states (Clusters 1 and 2), consistent with an active T cell response (Fig. 4d) involving naïve, cytotoxic, and activated/exhausted T cells in proportions consistent with ongoing tumor immunoediting.