Box plot indicated that significant alterations were observed in 14 immune cell populations between the normal and HF groups, including B cells naive, Plasma cells, T cells CD8, T cells CD4 naive, T cells CD4 memory resting, T cells CD4 memory activated, T cells follicular helper, NK cells activated, Monocytes, Macrophages M0, Macrophages M1, Macrophages M2, Mast cells resting, and Neutrophils (Figure 7B). The gene discussed is CD8A; the disease is hydrops fetalis.