A study on microbubble-mediated focused ultrasound immunoregulation in human T cells demonstrated that this method alters the concentrations of key analytes, including IL-1β, TNF-α, TNF-β, CCL21, CX3CL1, and soluble CD40L, by modulating critical signaling pathways in the immune response, such as NFκB and TNF pathways, thus enhancing the efficacy of cancer immunotherapy (123). The gene discussed is TNF; the disease is cancer.